Identification and distribution of gynogenetic triploid clones in ginbuna Carassius langsdorfii collected from Hiroshima and Yamaguchi prefectures [Japan]
2001
Maeda, K. (Hiroshima Univ., Fukuyama (Japan). Faculty of Applied Biological Science) | Umino, T. | Morishima, K. | Maemura, H. | Zhang, Q. | Arai, K. | Nakagawa, H.
Triploid ginbuna is a unisexual species and reproduces gynogenetically as well as clonally. A total of 312crucian carp were collected from Ohta River, Kurose R., Nuta R., Hakuryu Lake, and Ashida R. in Hiroshima Prefecture and Nishiki R. in Yamaguchi Prefecture. Ploidy status of these samples was determined by DNA content flow-cytometry and/or erythrocytes measurements and all-female triploid ginbuna were detected in frequencies between 36 and 100%. Multilocus DNA fingerprinting identified 16 clonal lines based on the genetic identity between at least two samples. An individual specific DNA fingerprints was detected in 56 samples, suggesting the presence of independent 56 and more clonal lines in the localities. Among 16 lines, six lines were commonly found in two or three localities, while the other 10 lines were secific to each locality. Predominant clonal line was different among the localities and it was also different among year classes even in the samples from the same locality.
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