Harpin(psta) from Pseudomonas syringae pv. tabaci is defective and deficient in its expression and HR-inducing activity
2001
Taguchi, F. (Okayama Univ. (Japan). Faculty of Agriculture) | Tanaka, R. | Kinoshita, S. | Ichinose, Y. | Imura, Y. | Andi, S. | Toyoda, K. | Shiraishi, T. | Yamada, T.
To elucidate the role of harpins produced by Pseudomonas syringae, the corresponding hrpZ gene was isolated from P. s. pv. tabaci. The sequence information revealed that this gene carries a serious mutation with 326 bp lacking in the central region and potentially encodes only 140 N-terminal amino acids because of a frame shift. The investigation of biological properties using recombinant harpin indicated harpin sub(psta) was incapable of inducing HR in both host and nonhost plants. Based on an immunoblot analysis to detect harpin from P. s. pathovars in hrp-inducing medium, the truncated harpin sub(psta) was neither expressed nor secreted into the culture medium. These results suggest that harpin is not the sole determinant of the host-parasite specificity in P. s. pv. tabaci.
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