Nucleotide sequencing of bovine alpha 4 integrin cDNA, and its expression in the primarily-cultured skeletal muscle satellite cells
2001
Muroya, S. (National Inst. of Livestock and Grassland Science, Kukizaki, Ibaraki (Japan)) | Nakajima, I. | Chikuni, K.
Alpha 4 integrin has been known to play an important role in the myotube formation of mouse skeletal muscle satellite cells on the myotube surface. In the present study, the 3480-bp nucleotide sequence of the bovine alpha4 integrin cDNA was determined, including the entire coding region of 1033 amino acids. The bovine alpha4 integrin cDNA has 81.4% and 87.2% identity with the mouse and human alpha4 integrin nucleotide sequences, respectively. The deduced amino acid sequence of the bovine alpha4 integrin mature protein showed 81.1% and 85.9% identity with the mouse and human alpha4 integrin amino acid sequence, respectively. An RT-PCR analysis showed that bovine alpha4 integrin was expressed in both the proliferating and differentiating primarily-cultured skeletal muscle satellite cells at higher levels than in the muscle tissue. This expression before the differentiation suggests that alpha4 integrin plays a role in proliferating mononucleated satellite cells in skeletal myogenesis.
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