Effects of heat and high-pressure treatments on antigenicity of beef extract
2002
Han, G.D. (Niigata Univ. (Japan)) | Matsuno, M. | Ikeuchi, Y. | Suzuki, A.
The sera of bovine gamma globulin (BGG) positive beef allergic patients were used in this study in order to investigate changes in IgE-specific binding activity with regard to beef extract altered by heat or high-pressure treatment. In inhibition-ELISA, the sample treated at 60degC did not show any significant changes in the antigenicity of BGG, but the sample treated at 100degC showed a decrease of the antigenicity. ln the case of the treatment with heating at 100degC, heat-coagulation occurred in the beef extract. The resulting supernatant and precipitate of the sample by centrifugation were analyzed by immunoblotting. Only the fraction of precipitate showed a specific binding activity with the sera. Based on this result, it was speculated that the persistent antigenicity found even after the treatment at 100degC in inhibition-ELISA remained principally in the heat-coagulated fraction, which indicated the importance of the method of handling the heat-coagulation in heat treatment. High-pressure treatments (200 MPa- 600 MPa) of beef extract did not show any significant changes in the binding with the sera.
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