Studies on the efficient production of in vitro fertilized bovine embryo
2000
"Shimada, H. (Mie-ken. Agricultural Technical Center, Ureshino (Japan)) | Sakakibara, H. | Nishi, Y. | Yotani, Y. | Itou, H. "
"To improve the development rate and the survivability of in vitro fertilized bovine embryo, we firstly examined on the development to blastocyst from embryo on the three kinds of synthetic mediums. The rate of embryonic development on the media, m-SOF and CR1aa, exceeded that TCM199. The cell number of embryo did not differ significantly among three kinds of mediums. After de-freezing, survival rate of embryo was higher in 7-day of age than in 6-day and 8-day in age. Secondly, several growth factors were added to synthetic medium. TGF alpha and EGF were added to the maturation medium with TCM199. And the growth factors TGF beta and FGF were added to the development medium, m-SOF. No significant difference was found in developmental rate, the cell number of embryo and survival rate after the de-freezing between the plots with and without adding grouth factors."
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