Peroral infection of nucleopolyhedrovirus non-occluded viral particle of Bombyx mori L. aided by a fluorescent brightener
2002
"Arakawa, T. (National Inst. of Agrobiological Sciences, Tsukuba, Ibaraki (Japan)) | Furuta, Y. | Kubomura, Y. | Kato, M. | Hayasaka, S. "
"A procedure to perorally infect the silkworm B. mori with nucleopolyhedrovirus (NPV) non-occluded viral particles was developed. The silkworm larvae were fed an artificial diet containing Tinopal UNPA-GX (Tinopal), a fluorescent brightener, for 24 h, then fed a diet contaminated with the non-occluded viral particle of B. mori NPV (BmNPV). A hemolymph supernatant of silkworm larvae infected with BmNPV from which the viral polyhedra had been removed was used as an original inoculum. When the silkworm larvae were fed a diet with 0.3% (w/w) Tinopal (1400g/1000 larvae) and administered an inoculum that was diluted one-thousand times (50 ml/600g diet/1000 larvae), all the larvae died of viral infection. A diet containing 0.3% Tinopal which had been autoclaved (121degC, 20 min) and stored at 5deg C for 300 days did not lose its activity to enhance viral infection. This newly developed peroral inoculation method of non-occluded BmNPV particle utilizing Tinopal will lead to industrial pharmaceutics production for using a baculovirus vector in a huge number of insect hosts. National Institute of Agrobiological Sciences (NIAS), Ohwashi 1-2, Tsukuba-shi, Ibaraki 305-8634, Japan."
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