Studies on the embryogenic processes in the in vitro culture of wheat [Triticum aestivum] somatic tissues by using a proliferative antigen of initial cells
2002
Evseeva, N.V. (Russian Academy of Sciences, Saratov (Russian F.)) | Tkachenko, O.V. | Lobachev, Y.V. | Mitrophanov, A.Y. | Djatchouk, T.I. | Shchyogolev, S.Y.
Embryogenic processes occurring in an in vitro culture of immature wheat (Triticum aestivum L.) embryos were studied using the proliferative antigen of initial cells, PAI - a cell-division-associated molecular marker for apical meristematic cells of cereal crops. With an immunochemical test-system based on polyclonal, monospecific anti-PAI antibodies, we found a correlation between PAI content and the embryogenic potential of calli of the wheat cultivar Saratovskaya 29 and its near-isogenic lines differing in the Rht-B1c alleles. We discuss the possible association of PAI with the Rht genetic system and the prospects for using this marker in breeding and bioengineering programs to screen newly raised wheat lines for their embryogenic capacity.
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