Studies on the soil mycoflora of Karachi coast [Pakistan]
2004
Manzoor, S. | Dawar, S. | Shaukat, S.S.,Karachi Univ. (Pakistan). Dept. ofBotany
Sixteen soil samples were collected from different localities of Karachicoast. Soil samples were analyzed for soil texture, pH, water content, organiccarbon, total nitrogen content, electrical conductivity and for the study of soilmycoflora. Almost all samples showed a sandy texture except that of Hub soilwhich showed clay loam texture. pH of the soil samples ranged between 7.9-8.1,organic carbon 1.4-6.6%, nitrogen content 0.98-2.38%, water content 0.8-14% andelectrical conductivity of soil samples were 1.05- 75.2 mS/cm. Mycoflora of soilsamples was detected by different techniques viz., baiting technique forisolation of Rhizoctonia solani, direct plating technique, serial dilutiontechnique and wet sieving for the isolation of Macrophomina phaseolina.Twenty eight species of 16 different genera were isolated and identified.Results showed that highest numbers of fungi were isolated by soil dilutiontechnique whereas, lowest number of fungi by wet sieving technique.Aspergillus niger and A. flavus exhibited highest frequency of occurrence byall 4 techniques. Aspergillus terreus, Rhizopus stolonifer and Syncephalastrumracemosum also appeared as dominant fungi from all the soil samples. Baitingtechnique resulted in significant isolation of R. solani whereas results of wetsieving did not yield sclerotial structures of M. phaseolina.
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