Micro-propagation of guava through shoot culture
2004
Raziuddin | Shah, S.S. | Ali, F.,NWFP Agricultural Univ., Peshawar(Pakistan). Dept. of Plant Breeding and Genetics
In vitro guava propagation through meristem culture was carriedout at Nuclear Institute for Food and Agriculture (NIFA), Peshawarduring 2002. The effect of different concentrations of certainsterilents with different treatment durations on meristem tips ofguava cultivar (cv) `Sufeda' used as explants, was evaluated forcontamination reduction. In the present study, mercuric chloride(HgCl2,) with 0.05% concentrations for five minutes was found moreeffective (67%) in reducing contamination of shoot tip culture bymicrobial growth, while the treatment having calcium hypochlorite(NaHCI) with 4 concentration for 5 minutes gave lowest survivalpercentage (25%). An antioxidant solution containing citric acid andascorbic acid (75:50 mg/I) gave maximum number of green shoot tips(55%), whereas minimum number of green shoot tips (16%) wereobtained from Murashige Skoog (MS) medium having no antioxidant.Highly shoot development percentage (72%) was recorded for MSmedium supplemented with 1.0 mg/l BAP along with 500 mg/lglutamine. The highest root development (77%) was observed on theMS medium supplemented with 2.5 mg/I IAA + 2.5 mg/l IBA, whereas,the medium supplemented with 2 mg/I of IAA initiated minimum rootgrowth. In light of all the results it is concluded that HgCl2 with0.05% concentration for five minutes was optimal for microbial freeshoot tips, while the combination of citric acid and ascorbic acid(75:50 mg/I) for healthy shoot tips, 2.5 mg/I of BAP for shootdevelopment, and the combination of 2.5 mg/I each IAA and IBA wereoptimal for rooting in guava cv `Sufeda'.
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