Effect of beta-1.3/1.6 glucan in diets on the effectiveness of anti-Yersinia ruckeri vaccine an experimental study in rainbow trout (Oncorhynchus mykiss)
Siwicki, A.K. | Kazun, K. | Glabski, E.(Inland Fisheries Institute, Olsztyn (Poland). Dept. of Fish Pathology and Immunology) | Terech-Majewska, E.(University of Warmia and Mazury, Olsztyn (Poland). Dept. of Epizootiology) | Baranowski, P. | Trapkowska, S.(University of Warmia and Mazury, Olsztyn (Poland). Dept. of Microbiology and Clinical Immunology)
The influence of beta-1.3/1.6 glucan, a natural product, on the antibody secreting cells (ASC) and specific Ig levels after immunization of rainbow trout (Oncorhynchus mykiss) with the anti-Yersinia ruckeri vaccine was studied. Fish were fed pellets containing beta-1.3/1.6 glucan (Macrogard) at a dose of 0.5 g/100 g of pellets (0.5 percent) per day. After one week of beta-1.3/1.6 glucan supplementation, the fish were immunized by immersion of the vaccine. At 7, 14, 21, 28 and 40 days after the immunization, blood and pronephros were taken from 20 fish in each group for immunological study. When analysed by the ELISPOT assay, beta-1.3/1.6 glucan (Macrogard) was found to increase the number of specific antibody secreting cells (ASC) and specific Ig levels in serum. In conclusion, the results of the present study showed that beta-1.3/1.6 glucan (Macrogard) increased the effectiveness of anti-Yersinia ruckeri vaccine in fish
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