QTL analysis for discoloration of flag leaves during the ripening period in rice
2003
Obara, M. | Fukuta, Y. | Yano, M. | Yamaya, T. | Sato, T.
Our study aimed to identify the putative quantitative trait loci (QTLs) associated with the discoloration of flag leaves during the ripening period, using 98 BC1F6 lines derived from Nipponbare (japonica)/Kasalath (indica). We used 245 restriction fragment length polymorphism markers to construct a framework linkage map. We measured the chlorophyll content of flag leaves by the SPAD chlorophyll meter (Minolta Co. Ltd., Tokyo, Japan) every 7 d within 5 wk from flowering. These SPAD values were used for the maximum content of chlorophyll and the half-life period of chlorophyll content. QTL analysis was conducted using interval analysis (QGENE). The half-life period of chlorophyll content of Kasalath was shorter than that of Nipponbare. Two putative QTLs associated with maximum chlorophyll content were mapped on chromosomes 6 and 9. A QTL associated with maximum chlorophyll content on chromosome 6 was close to the region associated with flowering date. Three putative QTLs associated with the half-life period of chlorophyll content were mapped on chromosomes 3, 6, and 10. A QTL associated with the half-life period of chlorophyll content on chromosome 6 coincided with the region associated with grain weight.
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