Selection of high green-plant regenerating lines through rice anther culture
2003
He, T. | Luo, K. | Han, S.H. | Guo, X.X.
Anthers from 32 crosses derived from indica x indica, indica x japonica, and indica x javanica were cultured. The number of inoculated anthers was about 71,600. All test materials could induce calli and regenerate green plants. Genotypic differences in anther culture ability were observed among the materials. Highly significant differences were observed in anther culture ability between indica x indica and indica x javanica. A significant negative relationship between callus induction and regeneration capacity was found. Both were positively correlated with green-plant yield. A new material from the indica/japonica hybrid progeny was screened through anther culture. Its callus induction frequency was higher, with a green-plant regeneration frequency of up to 97%, while the albino plant frequency was less than 2.2%. Green-plant regeneration capacities of calli induced from different induction media were not significant.
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