Phenotypic diversity in embryo mutants induced by tissue culture in rice
2003
Iwamoto, T. | Hong, S.K. | Imai, H. | Matsuoka, M. | Kitano, H.
We identified 146 rice embryo mutants from 6,000 M2 lines that had been regenerated from 3-mo-old Nipponbare calli. Among them, 52 mutants were characterized into three groups based on their phenotypes: deletion of embryonic organ(s), abnormal embryo size, and aberration in organ morphology. Mutants with deletion of embryonic organ(s) are composed of small globular embryos, club-shaped embryos, large undifferentiated embryos, and shootless and radicleless embryos. Changes in embryo-size mutants seemed to be in the size of their scutellum. Such variation in mutants detected in embryogenesis suggested that many genes could be related in regulating embryogenesis. The copy number of retrotransposon Tos17 increased in several mutants. Some mutations may be affected by Tos17 because it can transpose throughout the chromosome during tissue culture. Thus, we consider tissue culture useful for studying genetic regulation of organ differentiation and for isolating genes expressed during embryogenesis.
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