Shoot regeneration from anther culture of peanut in vitro
2001
Siriporn Pongsupasamit | Kanita Kumpeerapang | Chalit Pongsupasamit
Two experiments were conducted to induce callus and shoot regeneration from the cultured anthers of seven peanut cultivars. In the first experiment, the excised anthers at the uninucleate microspore stage were cultured on four different callus induction media. It was found that all four media were equally effective in inducing callus formation of all seven cultivars. Callus formation was best in Khon Kaen 60-4. The analyses of variances for response after 30 days of culture indicated the main effects of cultivar and the interaction effects of medium x cultivar were significant (P=0.01) while the main effects of medium were not significant. The analyses of variances for number of days to anther callusing and callus weight 30 days after culture indicated the main effects of medium and cultivar were significant (P=0.01) but the interaction effects were not significant. While the main effects of medium (A) and cultivar and interaction effects for callus growth weight were not significant (P0.05). In the second experiment, the anther-derived calli of the seven cultivars were transferred to five different shoot regeneration media. Twenty percent of the regenerated shoots were obtained from the anther-derived calli of the three cultivars, Tainan 9, Khon Kaen 60-1, Khon Kaen 60-2, cultured on N6+2 mg/L IAA+2 mg/L Kinetin + 2 mg/L GA3 medium.
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