Deterioration of soybean [Glycie max (L.) Merr.] seed stored under-low and high relative humidity: Changes in germination, vigor, membrane permeability and growth of storage fungi
2000
Arom Sripichit | Jarichart Kodedongkeng
Understanding the cause of soybean ([Glycine max (l) Merr.] seed deterioration during storage will aid us in devising methods to detect, maintain, and improve stand establishment. This study was designed to investigate early deteriorate changes of stored soybean seeds. To explore the mechanism of ageing, high quality seeds of the cultivar SJ5 harvested at physiological maturity were stored at room temperature with 5 moisture contents (6, 8, 10, 12 percent and variable moisture). Seed quality (germination and vigor), leakage conductivity, water absorption, staining with Evans blue and triphenyl tetrazolium chloride (TTC) and fungal infection were measured every 300 days during storage. Germination and vigor remained high only in seeds containing 6 percent moisture content throughtout storage, where as seeds with 8 percent showed small changes in the quality. Conductivity of seed leakage and water uptake increased for all seed moisture contents; however, this increment occurred early during storage prior to changes in seed quality of 6 percent seed moisture content. Staining seeds with Evans blue and TTC encouraged the changes in membrane integrity causing seed leakage which preceded measurable declines in seed quality. In addition, increase of fungi invasion did not associate with loss of seed quality. These results indicate that changes in membrane integrity appeared to be the first stage in seed deterioration.
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