Diagnosis of canine distemper disease using RT-PCR
2000
Weerawan Tiwananthakorn(Chiang Mai Univ., Chiang Mai (Thailand). Faculty of Veterinary Medicine) | Sirichai Junsuwanaruk(Chulalongkorn Univ., Bangkok (Thailand). Faculty of Veterinary Sciences. Dept. of Veterinary Pathology) | Anudep Rungsipipat(Chulalongkorn Univ., Bangkok (Thailand). Faculty of Veterinary Sciences. Dept. of Veterinary Pathology) | Achariya Sailasuta(Chulalongkorn Univ., Bangkok (Thailand). Faculty of Veterinary Sciences. Dept. of Veterinary Pathology) | Somporn Techangamsuwan(Chulalongkorn Univ., Bangkok (Thailand). Faculty of Veterinary Sciences. Dept. of Veterinary Pathology) | Sapasiri Siripornprasert(Chulalongkorn Univ., Bangkok (Thailand). Faculty of Veterinary Sciences. Dept. of Veterinary Pathology) | Tateyama, Susumu(Miyazaki University, Miyazaki (Japan). Faculty of Agriculture. Dept. of Veterinary Pathology) | Yamaguchi, Ryoji(Miyazaki University, Miyazaki (Japan). Faculty of Agriculture. Dept. of Veterinary Pathology) | Uchida, Kazuyuki(Miyazaki University, Miyazaki (Japan). Faculty of Agriculture. Dept. of Veterinary Pathology)
Diagnosis of canine distemper (CD) by a reverse transcriptase polymerase chain reaction (RT-PCR) technique using 2 sets of primer which were specific to two regions on the cDNA of nucleocapsid protein (NP) gene of the canine distemper virus (CDV), Onderstepoort strain. Together with pathology and immunohistochemistry, the RT-PCR test was used to confirm the diagnosis of canine distemper in 20 dogs. Histopathological examination revealed that 70 percent (14/20) had pneumonia, 50 percent (10/20) had non-suppurative encephalitis, 33 percent (7/20) had splenic follicular necrosis and 35 percent (7/20) follicular necrosis and 35 percent (7/20) had gastroenteritis. Immunohistochemical detection of CDV antigens in the brain, lungs, and the gastrointestinal mucosa were 85 percent (17/20), 58 percent (11/19) and 40 percent (6/15), respectively. In addition, the positive detection of amplified NPgene by RT-PCR from the brain, lungs, and gastrointestinal tissues were 100 percent (18/18), 83 percent (15/18) and 75 percent (12/16), respectively. Necropsy diagnosis made by histopathology and immunohistochemistry corresponded to the positive results shown by RT-PCR.
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