Study on enzyme keratinase from Bacillus licheniformis and B. pumilus in feather digestibility used as source of protein in animal feed
1999
Sunee Nitisinprasert (Kasetsart Univ., Bangkok (Thailand). Faculty of Agro-Industry)
On the basis of physiological and biochemical test using API LAB II plus analysis, two feather-degrading bacteria, KUB-K0006 and KUB-K0082, were classified as Bacillus Licheniformis and B. pumilus respectively. Synergistic reaction of crude enzymes fluid from KUB-K0006 (CFL) and KUB-K0082 (CFP) with the ratio of 5:1 showed higher digested feather than CFP and CFL alone for 3.04 and 2 times respectively. In addition the ratio of 1:5 showed higher amino acid released contents than CFP and CFL alone for 1.13 and 1.77 times respectively. By the way addition of 5 times CFL created the values of percent digestibility to be 1.4 and 1.2 times of the action of CFP and CFL alone respectively. When the suitable amount of enzyme was studied, 500-1000 units of CFL:CFP, 5:1, hydrolyzing feather provided percent digestibility more than 75 percent. Comparing to the commercial enzyme allzyme BPD, the comparative equally percent pepsin digestibility obtained showed no significantly difference when 1,000 units was used.
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