In vitro conservation and propagation of some rare species of plant
1999
Sureeya Tantiwiwat | Malee Na Nakorn | Srisom Surawattananon | Kamonpun Namwongprom (Kasetsart Univ., Bangkok (Thailand). Faculty of Science. Department of Botany)
Yaing shoots of Kaew Na ma Thalaybun (Alocasia lobbianum) were surface sterilized in chlorox solution two times. Leaf sheaths were peeled out before excise the terminal and lateral buds and placed on MS with free hormone for 7 days to check contamination percentage which were 0 percent. Then transferred the explants to MS supplemented with different BA concentrations for shoot multiplication. The result showed that MS with BA 8.0 ppm. Gave the highest shoot number which were 3.5 shoots per piece of explant. The shoots were induced roots on MS with NAA at different concentrations and showed that MS at 0.5 ppm. Of NAA gave the highest root number which were 16.5 roots per shoot with the length of 16.8 c.m. when cultured for 45 days. The plants were then transplanted to the growing medium and placed under mist for 20 days. Kaew Na Ma Thalaybun were 100 percent viable.
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