Changes in levels of some innate immune response parameters, blood vitamins E and A in allions, following castration
2005
Gundasheva, D. (Trakia University, Stara Zagora (Bulgaria)) | Khubenov, Kh. (Trakia University, Stara Zagora (Bulgaria)) | Kostov, K. (Regional Diagnostic Veterinary Institute, Stara Zagora (Bulgaria)) | Karadzhov, T. (Trakia University, Stara Zagora (Bulgaria))
We examined in stallions the impact of castration on total differential count of leukocytes and the classical pathway of complement activation (CPCA), blood vitamins A and E levels. The stallions were castrated with an emasculator, while in standing position, using the open technique. The number of leucocytes was counted in the Burker chamber, and leukogram on blood smear.The neutrophyl/lymphocyte ratio (N/L) was counted; CPCA was shown with Mayer's method adapted for horses.Vitamins E and A concentrations were determined spectrophotometrically. The castration led to an increase in neutrophyl bands number 3 hours later and on days 3 and 7; monocytes band number increased also 3 hours post castration. The applied castration caused temporary and quantitative changes in the examined parameters of innate defence by suppressing its humoral component - complement (0 hour) and caused an increase of the cell elements - neutrophils band and monocytes an d the N/L ratio. It led to E hypovitaminosis.
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