In vitro propagation of Tacitus bellus
2005
Mitrovic, A. (Center for Multidisciplinary Studies, Belgrade (Serbia and Montenegro)), E-mail: [email protected] | Vinterhalter, B. | Culafic, Lj.
The general aim of this study was to develop a suitable method for propagation of Tacinus bellus (fam. Crassulaceae). Three-year old plants 5 cm in diameter were used in experiments. Sterilized lower rossete leaves were aseptically placed on medium containing mineral and vitamin solution supplemented with sucrose (3%), benzylaminopurine (BAP) (2.25 mg/cubic dm) and naphtalenacetic acid (NAA) (0.186 mg/cubic dm) and gelled with agar (0.7%), Murashige and Skoog, 1962. Callus and a large number of buds were observed on the explant bases after six months of cultivation on the two-phase medium. The bud multiplications were performed on MS media supplemented with BAP (0.1-0.5 mg/cubic dm) and NAA (0.1 mg/cubic dm), while rooting of small rosettes were performed on NAA (0.1-0.2 mg/cubic dm). Spontaneous rooting (80%) were noticed on hormone free medium, while NAA (0.1 or 0.2 mg/cubic dm) stimulated it up to 100%, depending on clones. Rooted plants were easily acclimatized.
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