Genetic modification of horse chestnut as a tool for the ornamental traits improvement
2005
Zdravkovic-Korac, S., E-mail: [email protected] | Calic, D. | Radojevic, Lj. (Institut za bioloska istrazivanja Sinisa Stankovic, Beograd (Serbia and Montenegro))
Horse chestnut was transformed by using the agropine Agrobacterium rhizogenes strain A4GUS, with the uidA reporter-gene inserted in the TL-DNA. Plantlets were regenerated from the acquired hairy root lines via the process of somatic embryogenesis, and, to a lesser extent, organogenesis. Transformed plantlets displayed the characteristic hairy root syndrome at varying degrees. Plants of all lines were shorter than control ones, although branching did not increase. The line 36 had almost normal leaf morphology, while lines 43 and 31 had narrow leaves. Lines 39 and 47 displayed the most severe phenotype: stunted growth, very narrow dark-green leaves and short petioles. It seems that at least line 36 may be interesting, for example, for the bonsai plants production.
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