Effect of gamma-radiation on development, yield and quality of microtubers in vitro in Solanum tuberosum L.
Li, H.Z.(Zhejiang Univ., Hangzhou (China). Inst. of Crop Science) | Zhou, W.J.(Zhejiang Univ., Hangzhou (China). Inst. of Crop Science)E-mail:[email protected] | Zhang, Z.J.(Zhejiang Univ., Hangzhou (China). Inst. of Crop Science) | Gu, H.H.(Zhejiang Univ., Hangzhou (China). Inst. of Crop Science) | Takeuchi, Y.(Utsunomiya Univ. (Japan). Center for Research on Wild Plants) | Yoneyama, K.(Utsunomiya Univ. (Japan). Center for Research on Wild Plants)
Explants obtained from in vitro-propagated plantlets of two potato cultivars, Shepody and Atlantic, were treated with five doses of gamma-radiation (0, 2, 4, 6 and 8 Gy) to investigate the stimulating effects of low irradiation on the production and quality of microtubers in vitro. Microtubers of both cultivars treated with gamma-radiation initiated 5 d earlier than in the non-irradiated control. The whole period of microtuberization was prolonged by 10-15 d with 4, 6 and 8 Gy irradiation treatment for cv. Atlantic. Irradiation of the plantlets (4 Gy) led to a significant increase not only in the microtuber number (116.7 and 34.5% over the control) but also in the fresh mass (77.6 and 23.2% in Shepody and Atlantic, respectively). Low dose irradiation (2-4 Gy) increased the starch concentration of microtubers. High doses (6-8 Gy) enhanced ascorbic acid and reducing sugar concentrations. Protein concentrations were increased by 4-6 Gy doses.
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