Thidiazuron stimulates adventitious shoot regeneration in different safflower explants
2006
Radhika, K.(Acharya N.G. Ranga Agricultural Univ., Hyderabad (India)) | Sujatha, M.(Directorate of Rice Research, Hyderabad (India)) E-mail:[email protected] | Nageshwar Rao, T.(Acharya N.G. Ranga Agricultural Univ., Hyderabad (India))
Adventitious shoot regeneration from root, hypocotyl, cotyledon and primary leaf explants of Carthamus tinctorius was studied. Shoot regeneration was promoted by benzyladenine (BA) + naphthaleneacetic acid (NAA), BA + indole-3-butyric acid, kinetin + NAA and thidiazuron (TDZ) + NAA incorporated in Murashige and Skoog (MS) basal medium. High frequency of shoot regeneration and high number of shoots per regenerating explant were obtained on a wide range of TDZ + NAA combinations. Proliferated shoots were elongated in MS + 0.5 mg/cubic dm kinetin and well-developed shoots were rooted in half strength MS + 0.5 mg/cubic dm NAA. Rooted shoots were successfully acclimatized and established in soil.
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