Antioxidant defense mechanism under salt stress in wheat seedlings
2006
Mandhania, S.(CCS Haryana Agricultural Univ., Hisar (India). Dept. of Plant Breeding) | Madan, S.(CCS Haryana Agricultural Univ., Hisar (India). Dept. of Plant Breeding) E-mail:[email protected] | Sawhney, V.(CCS Haryana Agricultural Univ., Hisar (India). Dept. of Plant Breeding)
The effect of salt stress on cell membrane damage, ion content and antioxidant enzymes was studied in wheat seedlings of the salt-tolerant KRL-19 and salt-sensitive WH-542. Seedlings (4-d-old) were irrigated with 0, 50 and 100 mM NaCl. The relative water content declined with induction of salt stress, more in WH-542 than in cv. KRL-19. K+/Na+ ratio in KRL-19 was higher than in WH-542. WH-542 suffered greater damage to cellular membranes due to lipid peroxidation as indicated by higher accumulation of H2O2, malondialdehyde and greater leakage of electrolytes than KRL-19. The activities of catalase, peroxidase, ascorbate peroxidase and glutathione reductase increased with increasing salt stress in both cultivars, however, superoxide dismutase activity declined. Upon desalanization, partial recovery in the activities of these enzymes was observed in KRL-19 and very slow recovery in WH-542.
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