Efficient regeneration system from wheat leaf base segments
2006
Haliloglu, K.(Atatuerk Univ., Erzurum (Turkey). Dept. of Field Crops) E-mail:[email protected]
The purpose of our study was to develop a simple, reproducible and reliable in vitro regeneration system from wheat leaf base segments. The factors affecting the callus formation and regeneration capacity of leaf segments of two genotypes, Bobwhite and Pavon 76, were investigated. The highest number of somatic embryos (SE) was obtained on Murashige and Skoog medium supplemented with 2 mg/dm3 2,4-dichlorophenoxyacetic acid + 1 mg/dm3 naphthalenacetic acid (14.7 SE per segment). The highest frequencies of embryogenic callus (96%) and somatic embryo formation (24.3 SE per segment) were achieved in the first segments. The highest plantlet regeneration was obtained after transfer of embryogenic calli to regeneration medium supplemented with 1 mg/dm3 kinetin (6.3 plantlets per segment).
显示更多 [+] 显示较少 [-]