Influence of CCC, putrescine and gellam gum concentration on gynogenic embryo induction in Allium cepa
2006
Ponce, M.(National Univ. of Cuyo, Chacras de Coria (Argentina). Plant Physiology Lab.) E-mail:[email protected] | Martinez, L.(National Univ. of Cuyo, Chacras de Coria (Argentina). Plant Physiology Lab.) | Galmarini, C.(National Univ. of Cuyo, Chacras de Coria (Argentina). Inst. of Horticulture)
The induction of haploid plants by in vitro gynogenesis is a promising practice in onion breeding. In order to increase the frequency of embryo regeneration and haploid plant production in Valcatorce INTA, Cobriza INTA and Navidena INTA cultivars, putrescine and CCC were used, either as a component of the culture media or by spraying or injecting them to the umbels. Additionally, two concentrations of gellam gum were tested. A high number of gynogenic embryos was achieved by using 7 g/dm3 gellam gum. This number was not affected by the addition of putrescine to the media. CCC sprayed at the umbels significantly increased the gynogenic embryo rate which was more than three times higher than the control. Cobriza INTA showed the highest induced embryo rate (4.76%).
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