Effects of short light-dark regimes on in vitro shoot rooting of some fruit tree rootstocks
2006
Morini, S.(Universita di Pisa (Italy). Dipartimento di Coltivazione e Difesa delle Specie Legnose "G. Scaramuzzi") E-mail:[email protected] | Perrone, S.(Universita di Pisa (Italy). Dipartimento di Coltivazione e Difesa delle Specie Legnose "G. Scaramuzzi")
Experiments were carried out to evaluate the effects of 4/2 light-dark cycles (4 h of light followed by 2 h of dark) on the rooting responses of shoots cultivated in vitro of the fruit tree rootstocks GF 677 (peach x almond hybrid), Mr.S. 2/5 (Prunus cerasifera), MM 106 (apple Northern Spy x Paradise M1) and BA 29 (Cydonia oblonga). Under this light regime rooting percentage of GF 677, Mr.S. 2/5 and MM 106 shoots reached 100% as in the control treatment (16/8), whereas in BA 29 shoots, short light-dark cycles increased rooting response by about 65%. Moreover, the shoots of all rootstocks submitted to the 4/2 cycle showed an appreciable increase in root number and length, and an earlier root emergence of about 4-5 d in comparison with the 16/8 cycle. Finally, rooting percentage of BA 29 shoots submitted to the 4/2 light regime and treated with 0.2 mg/dm3 indolebutyric acid was equal to that reported with 0.4 mg/dm3 IBA under the 16/8 regime, indicating that the former light regime also amplified the rhizogenic effect of auxin.
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