Identification of domestic glutinous rice cultivars by the PCR method using grains of 18 typical glutinous rice cultivars as sample and development of technology for detection of different kind grain incorporation in glutinous rice processed foodstuf#fs#
2004
Nakamura, S. (National Food Research Inst., Tsukuba, Ibaraki (Japan)) | Suzuki, K. | Haraguchi, K. | Yoza, K. | Okunishi, T. | Matsui, T. | Ishizaki, K. | Yoshii, Y. | Ohtsubo, K.
A part of rice for staple food and for crushing rice has been provided as raw materials of processed glutinous rice products. For this background, almost manufacturer can not select raw material rice. Therefore, the technology to warrant quality of raw material rice is indispensable. By PCR using newly developed STS primers, blended corn and barley to glutinous rice products were detected. A part of primers were designed based on reported DNA sequences of Zein and Hordein. And promising primers derived of GBSS were used to amplify the discriminative DNA bands. GBSS genes were extracted from agarose gels of electrophoreses after PCR and cloned into a PCR-XL-TOPO vector followed by sequencing. Therefore, there was a slight difference among corn, barley and rice in DNA sequences of GBSS genes. A part of these different base regions were used to design the primers. These STS primers were showed to be useful to differentiate corn, barley and glutinous rice. DNA extraction from rice craker has some difficulties, however, it became possible to extract and purify by improving 'enzyme method', which we reported previously and extracted DNA could be amplified by PCR. And cultivar identification by the PCR method was investigated using 18 typical domestic glutinous rice cultivars.
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