DNA and chemical analyses of commercial fly agaric-related products
2005
Maruyama, T.(National Inst. of Hygienic Sciences, Tokyo (Japan)) | Kawahara, N. | Fukiharu, T. | Yokoyama, K. | Makino, Y. | Goda, Y.
Since June 6, 2002, psilocin and psilocybin-containing fungi (commonly called "magic mushrooms") have been regulated by the Narcotics and Psychotropics Control Law m Japan However, various fly agaric-related products are now entering the Japanese market via the internet In this study, fly agaric-related products available m this way were investigated for raw materials by DNA analysts and for additives by chemical analysts Nucleotide sequence analysis of the mitochondrial 12S rDNA region suggested that these fly agaric-related products originate from A. muscaria or A muscarva var. persicina.. Furthermore, they were classified into three strains based on the ITS2-LSU nucleotide sequence Harmine derivatives and/or tryptamine derivatives were detected in some of these products by LC/MS analysis. In accordance with this, the matK gene of Peganum harmala was found m all of the harmine derivative-containing samples.
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