Effects of beta-mercaptoethanol on ATP contents in cumulus cell-enclosed bovine oocytes matured in vitro and sequential development of resultant embryos from in vitro fertilization
2005
Tsuzuki, Y.(Miyazaki Univ. (Japan). Faculty of Agriculture) | Saigoh, Y. | Ashizawa, K.
In the present study, we assessed the effects of beta-mercaptoethanol (beta-ME) added to in vitro maturation (IVM) medium on the development of resultant embryos derived from in vitro fertilization (IVF) of oocytes, the number of cumulus cells, and ATP content of bovine oocytes. Furthermore, we counted the number of cells of the blastocysts derived IVF of oocytes matured in media with or without 100 muM beta-ME. When beta-ME was added into the medium of IVM at concentrations of 0, 10, 50, 100 and 500 muM, the rate of embryonic development to the blastocyst stage of resultant embryos increased significantly (P0.05) in the 100 muM group compared to the 0 muM (control) group. Although beta-ME was added to media with the same concentrations (0, 10, 50, 100 and 500 muM) for the embryonic culture following in vitro fertilization of oocytes matured without beta-ME, the rates of embryonic development to the blastocyst stage decreased in a concentration-dependent manner. The number of cumulus cells attached to the oocytes matured in media with 100 muM beta-ME was significantly greater (P0.05) than that in the 0 muM group. There was no difference between ATP contents of cumulus cell-enclosed oocytes (CO) matured with or without 100 muM beta-ME. In contrast, the ATP content of cumulus cell-denuded oocytes after maturation in media supplemented with 100 muM beta-ME was significantly lower (P0.05) than that of the 0 muM-treated group. In addition, the average cell number of blastocysts in the 100 muM beta-ME group was significantly greater (P0.05) than that of the 0 muM group. These results suggest that beta-ME may increase both the numbers of cumulus cells attached to oocytes and the cell numbers of blastocysts derived from in vitro fertilization by influencing the ATP metabolism of bovine oocytes during IVM.
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