Inhibition of growth hormone secretagogue receptor antagonist, (D-Lys-3)-GHRP-6, in adipogenesis of ovine and rat adipocytes
2005
Roh, S.G.(Shinshu Univ., Minamiminowa, Nagano (Japan). Faculty of Agriculture) | Hong, Y.H. | Hishikawa, D. | Tsuzuki, H. | Miyahara, H. | Nishimura, Y. | Goto, C. | Suzuki, Y. | Choi, K.C. | Lee, H.G. | Sasaki, S.
Ghrelin and growth hormone secretagogues receptor (GHS-R or ghrelin receptor) have been reported as being one of the factors of adipogenesis in adipocytes. To investigate the involvement of ghrelin and GHS-R in adipocytes, the effect of the GHS-R antagonist, [D-Lys-3]-GHRP-6 (His-D-Trp-D-Lys-Trp-D-Phe-Lys-NH2), on the process of adipogenesis in ovine and rat adipocytes was evaluated. [D-Lys-3]-GHRP-6 (10(-7) mol/L) significantly inhibited adipogenic differentiation of ovine and rat preadipocytes prepared from adipose tissues. The level of peroxisome proliferator activated receptor (PPAR)-gamma(2) mRNA, an adipogenic marker, was decreased during the differentiation of adipocytes treated with [D-Lys-3]-GHRP-6 for 10 days. Ghrelin stimulated adipogenesis, also causing an increment of glycerol-3-phosphate dehydrogenase and upregulation of PPAR-gamma(2). Furthermore, the antilipolytic effect of ghrelin was attenuated by treatment with [D-Lys-3]-GHRP-6 in both types of isolated adipocytes. Overall, the results of the present study highlight that GHS-R in adipogenesis can be blocked by treatment with [D-Lys-3]-GHRP-6.
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