Evidence for estrogen receptor expression in the bursal epithelial cells of chicks
2005
Shin, Y.H.(Okayama Univ. (Japan). Faculty of Agriculture) | Takagi, F. | Sugita, S. | Han, S. | Tsuji, T. | Abe, A. | Kondo, Y.
The aim of the present study was to demonstrate estrogen receptor (ER) alpha (ER-alpha) and ER-beta expression in the bursa of Fabricius and cultured bursal epithelial cells (CBEC) by reverse transcription (RT)-polymerase chain reaction (PCR) using a primer set design based on the sequences of chicken ER-alpha complementary DNA (cDNA) and chicken ER-beta-cDNA. In the case of ER-alpha, RT-PCR products of the expected size (698 base pairs (bp)) were amplified from cDNA derived from the bursa and CBEC, and from the oviduct, which was used as a positive control. All of the products were cleaved into two fragments of 476 bp and 222 bp by treating the products with restriction nuclease Hind III, indicating that these RT-PCR products from the bursa, CBEC and genital organs were the same. In contrast, in the case of ER-p, PCR products of the expected size (303 bp) were not detected in the bursa of Fabricius or CBEC, or in the oviduct and ovary. These results indicate that ER-alpha is expressed in the bursa of Fabricius and in CBEC, but ER-beta is not expressed in the bursa or CBEC, or in the female genital organs of chickens.
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