Cell microarray for screening feeder cells for differentiation of embryonic stem cells
2005
Yamazoe, H.(Kyoto Univ. (Japan)) | Iwata, H.
Microarrays are currently recognized as one of major tools in the assessment of gene expression via cDNA or RNA analysis and are now accepted as a powerful experimental tool for highthroughput screening of a large number of samples, such as cDNA and siRNAs. In this study, we examined the potential of the microarray methodology for high-throughput screening of candidate cells as feeder cells which effectively differentiate embryonic stem (ES) cells to the specific lineage. Cell arrays were prepared by applying three kinds of cells PA , human umbilical vein endothelial, and COS-1 cells, to circular spots, 2mm in diameter, on a glass plate, followed by the application of mouse ES cells to the cell microarray, After 8 d in culture, TuJ1 (neuron-specific class 111 beta-tubulin) immunocytochemieal staining clearly demonstrated that only PA6 cell spots had the capability to induce ES cells to neuronal differentiation. Although this is a model experiment, these findings clearly indicate that the cell microarray will become a powerful tool for highthroughput screening large numbers of candidate feeder cells for specific differentiation.
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