Genetic transformation of 'Kyoho' grape with stilbene synthase gene
2006
Nakajima, I.(National Inst. of Fruit Tree Science, Tsukuba, Ibaraki (Japan)) | Kobayashi, S. | Matsuta, N. | Sato, A. | Yamada, M. | Soejima, J.
A stilbene synthase gene, isolated from Vitis riparia, controlled under the cauliflower mosaic virus 35S promoter (35SP), was introduced into 'Kyoho' grape (Vitis x labruscana) via the Agrobacterium-mediated method. Ninety-one regenerated plants were obtained. PCR analysis showed that 55 plants contained the 35SP-stilbene synthase gene. Stilbene content of 34 transformants was measured by HPLC. The transformants contained stilbene derivative instead of resveratrol, and piceid (resveratrol-glucoside) was the candidate for the derivative. The highest content of the stilbene derivative was 19.7 mug/g (fresh weight) among the transformants. Detached leaf test of transformants did not show disease resistance against gray mold (Botrytis cinerea).
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