Effects of aged garlic extract supplementation on urinary 8-OHdG content, NK-cells activity and POSM scores in clinical training for university students
2005
Kimoto, R.(Hokkaido Coll. of Arts and Science, Ebetsu (Japan)) | Kambayashi, I. | Uchida, E. | Kyo, E. | Takeda, H.
The purpose of this study was to examine whether aged garlic extract (AGE) supplementation influence or not the change of urinary 8-hydroxy-deoxyguanosine (8-0HdG) content natural killer (NK) -cells activity and profile of mood states (POMS) scores during clinical training. Ten healthy male university students were recruited and participated in clinical training for 7 weeks for becoming physiotherapists. They were divided into two groups, AGE supplementation group (S-group, n=5) and control group (C-group, n=5). S-group was supplemented with AGE during clinical training. Urinary 8-0HdG content was analyzed by enzyme linked immunosorbent assay before (pre), after progress for 20 days (middle) and after (post) clinical training. NK-cells activity, which was analyzed by a standard 4-hour chromium release assay and POMS test were measured at pre and post, respectively. In C-group, Urinary 8-0HdG content, NK-cells activity and POMS scores did not change during clinical training for 7 weeks. These results indicate that clinical training for 7 weeks does not bring about such a strong stress for subjects. Therefore, we thought that significant effects of AGE supplementation in urinary 8-0HdG content and NK-cells activity could not be obtained in S-group. With POMS test, high fatigue and confusion scores at pre (60.6+-5.2 and 64.2+-4.2) significantly decreased at post (44.6+- 2. 2 and 49.8+-3. 5) , respectively. However, it is not clear if this change depend ,on AGE supplementation or effect of practice over clinical training. Further research needs to clarify effect of AGE supplementation on physiological and psychological stress.
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