A Surgical Method for Collecting Canine Oocytes of In Vivo Matured from Uterine Tube
Kim, M.K.;Oh, H.J.;Fibrianto, Y.H.;Jang, G.;Kim, H.J.;Lee, K.N.;Kang, S.K.;Lee, B.C.;Hwang, W.S.(Seoul National University, Seoul, Republic of Korea)E-mail:[email protected]
Oocytes matured in vivo can be collected surgically from bitches and used as recipient for IVF and nuclear transfer. However, the number of oocytes that can be obtained using this approach is very limited, until now typically a mean of approximately four good oocytes per collection (Westhusin et al., 2001). But the result of present study indicate that it is possible to obtained high percentage and quality in vivo oocytes from bitch for IVF and cloning dogs using our invented bulbed needle. Salpingectomy have spent more time for handling on operating and resulted in increasing bleed and more invasive than the surgical methods used with bulbed needle ligation.
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