Activity of the Extracts from Polyporus umbellatus as a Apoptosis Inducer
Shin, Y.J.;Kim, B.S.;Suh, Y.J.;Cho, D.J.;Lee, Y.K.(Dongseo University, Busan, Republic of Korea)E-mail:[email protected] | Choi, W.E.(Daewoo Pharm. Co. Ltd., Busan, Republic of Korea)
The effect of gradient eluted fractions from Et₂O extracts of Polyporus umbellatus screlotium was investigated on the viability of leukemia cell lines, K-562, L-1210, HL-60 and U-937 cells. Among those fractions, fraction 2 showed mild cytotoxic effect on L-1210 and HL-60 cells. Fraction 3 showed cytotoxic effect on 4 cell lines, and cytotoxic effect was the most potent on L-1210. The hallmark of apoptosis, DNA fragmentation, also appeared by fraction 3 on L-1210 after 48 hr treatment. Furthermore, this fraction was shown to be able to induce cell death on L-1210 cells by the inhibition of DNA synthesis in [³H]thymidine incorporation test.
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