Effect of Growth Regulators on Asiaticoside Production in Whole Plant Cultures of Centella asiatica (L.) Urban
Kim, O.T.;Kim, M.Y.;Huh, S.M.;Hwang, B.(Chonnam National University, Gwangju, Republic of Korea)E-mail:[email protected] | Ahn, J.C.(Seonam University, Namwon, Republic of Korea) | Seong, N.S.(National Institute of Crop Science, RDA, Suwon, Republic of Korea)
We investigated the effects of growth regulators on whole-plant cultures derived from nodes of Centella asiatica. A B5 liquid medium including 0.01 mg L-¹ 2,4-D resulted in decreased growth and asiaticoside production. Among the cytokinins tested (TDZ, BA, zeatin, and kinetin), TDZ was the best supplement for the promotion of asiaticoside biosynthesis. To directly estimate this effect, we measured asiaticoside content in the leaf, the main organ for synthesis. The addition of TDZ did not affect asiaticoside accumulation.
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