Characterization of the α-Galactosidase Gene from Leuconostoc mesenteroides SY1
2005
Kim, J.H. (Gyeongsang National University, Jinju, Republic of Korea) | Park, J.Y. (Gyeongsang National University, Jinju, Republic of Korea) | Jeong, S.J. (Gyeongsang National University, Jinju, Republic of Korea) | Chun, J.Y. (Gyeongsang National University, Jinju, Republic of Korea) | Lee, J.H. (Kyonggi University, Suwon, Republic of Korea) | Chung, D.K. (Kyung Hee University, Seoul, Republic of Korea) | Kim, J.H. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: [email protected]
Leuconostoc mesenteroides SY1, an isolate from kimchi, was able to ferment α-galactosides, such as melibiose and raffinose. α-Galactosidase (α-Gal) activity was higher in cells grown on melibiose and raffinose than cells grown on galactose, sucrose, and fructose. α-Gal activity was not detected in cells grown on glucose, indicating the operation of carbon catabolite repression (CCR). A 6 kb DNA fragment was PCR amplified using a primer set based on the nucleotide sequence of a putative α-galactosidase gene (aga) from L. mesenteroides ATCC 8293.
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