Production and diagnostic applications of monoclonal antibodies against porcine circovirus
Kim, K.M.;Jeong, J.H.;Min, H.K.;Lee, S.C.;Kang, S.Y.(Chungbuk National University, Cheongju, Republic of Korea)E-mail:sykang@chungbuk.ac.kr | Roh, I.S.(National Veterinary Research and Quarantine Service, Anyang, Republic of Korea)
Porcine circovirus type 2 (PCV-2) has been associated with various disease in pigs worldwide including postweaning multisystemic wasting syndrome (PMWS) and porcine dermatitis and nephropathy syndrome (PDNS). In this study, monoclonal antibodies (MAbs) against PCV were produced, characterized and applications of MAbs as diagnostic reagents were described. Spleen or lymph node cells from BALB/c mouse immunized respectively with PCV-1, PCV-2 or expressed PCV-2/ORF2 proteins in baculovirus were fused with SP2/0 myeloma cells using polyethylene glycol (PEG) and hybridoma cells producing PCV-1 or PCV-2-specific antibody were screened by an indirect immunofluorescence (IIF) test.
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