Comparison of DNase activities from excretory/secretory products of Haemonchus contortus fenbendazole-resistant
Kwak, D.M.(Washington State University, Pullman, WA, USA)E-mail:dmkwak@vetmed.wsu.edu
Change in β-tubulin nucleic acid and protein sequences was the only known difference between Haemonchus contortus fenbendazole (FBZ)-resistant and -susceptible isolates. This change was sufficient to determine the pathologic effect induced by FBZ treatment. This research was initiated to investigate further differences from these two isolates. Since β-tubulin is involved in formation of microtubule, which has functions in secretory vesicle transport, DNase activities from excretory/secretory products (ESP) of the two isolates were compared, based on pH, sensitivity to DNase inhibitors, molecular masses and production of 3'-OH.
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