Detection of infectious canine hepatitis virus by TaqMan real-time PCR method
Wang, H.Y.;Choi, J.Y.;Lee, M.J.;Park, J.H.;Cho, M.R.;Han, J.C.;Choi, K.S.;Chae, J.S.(Chonbuk National University, Jeonju, Republic of Korea)E-mail:jschae@chonbuk.ac.kr
The aim of this work was the validation of a rapid real-time PCR assay based on TaqMan technology for the unequivocal identification of infectious canine hepatitis (ICH) virus, to be used directly on DNA purified from blood specimens. A real-time PCR system targeting at the E3 ORFA gene sequence of canine adenovirus type 1 was optimized and validated through comparative analysis of samples using conventional PCR system. The real-time PCR assay based on TaqMan technology could disclose 23 (37.7%) out of 61 samples as PCR positive. In contrast, 18 (29.5%) samples were found PCR positive when conventional PCR was applied on these samples.
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