Methylation Pattern Analysis of Transgenic Tobacco Plants
2004
Lim, C.W. (Kyung Hee University, Yongin, Republic of Korea) | Kim, H.S. (Kyung Hee University, Yongin, Republic of Korea) | Oh, Y.J. (Kyung Hee University, Yongin, Republic of Korea) | Park, Y.D. (Kyung Hee University, Yongin, Republic of Korea)
Tobacco (Nicotiana tabacum cv. Havana SR1) was transformed with Agrobacterium tumefaciens LBA4404 containing a rescue cloning vector (pRCV2). Southern hybridization data showed that the copy number of integrated genes varied from one copy to multiple copies. Transgenic plants confirmed with Southern hybridization were selfed and the T₁ seeds from the transgenic lines with various copy numbers were tested in hygromycin medium to examine the progeny segregation ratio.
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