Induction and Characterization of Tetraploid Watermelon
2004
Jaskani, Muhammad J. (Kochang Watermelon Experiment Station, Gochang,) | Koh, G.C. (Honam University, Gwangju, Republic of Korea) | Huh, Y.C. (National Horticultural Research Institute, RDA, Suwon, Republic of Korea) | Kwon, S.W. (Kochang Watermelon Experiment Station, Gochang,) | Ko, B.R. (Kochang Watermelon Experiment Station, Gochang,)
Diploid watermelon lines were treated with colchicine to develop tetraploid seed parents. Seedlings of ten diploid lines were raised and injected twice with colchicine (0.2, 0.4, and 0.6%) for 3 consecutive days at emergence of true leaves. Watermelon lines responded differently for colchicine concentrations but showed maximum mortality at 0.6% colchicine. The treated seedlings were evaluated on the basis of chloroplast count, DNA content and morphology. The chloroplast counts in each side of guard cells ranged 5-7 and 10-12 in diploids and tetraploids, respectively.
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