Biosynthesis of Cephalexin in PEG400-Ammonium Sulfate and PEG400-Magnesium Sulfate Aqueous Two-Phase Systems
2004
Cao, Xue Jun (East China University of Science and Technology, Shanghai, China), E-mail: [email protected] | Zhu, Jian Hang (East China University of Science and Technology, Shanghai, China) | Wei, Dong Zhi (East China University of Science and Technology, Shanghai, China) | Hur, Byung Ki (East China University of Science and Technology, Shanghai, China)
The biosynthesis of cephalexin was carried out in the aqueous two-phase systems using penicillin acylase as a catalyst, and 7-aminodeacetoxicephalosporanic acid (7-ADCA) and phenylglycine methyl ester (PGME) as substrates. 20% PEG400-l7.5% (NH₄)₂SO₄ containing 0.5 M NaCl and 1.5 M methanol aqueous two-phase systems (ATPS) were selected as reaction medium, and 53% product yield was obtained using immobilized penicillin acylase as a catalyst. 20% PEG400-l5% MgSO₄ ATPS was also used for the synthesis of cephalexin, and 60-62% product yield was obtained by using free penicillin acylase as a catalyst.
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