Isolation and Characterization of Transcriptional Elements from Corynebacterium glutamicum
2004
Park, S.D. (Korea University, Seoul, Republic of Korea) | Choi, J.S. (BASF Research Center, Kunsan, Republic of Korea) | Kim, Y.H. (Semyung University, Checheon, Republic of Korea) | Lee, S.N. (Korea University, Seoul, Republic of Korea) | Park, I.H. (Korea University, Seoul, Republic of Korea) | Lee, H.S. (Korea University, Seoul, Republic of Korea), E-mail: hlee@korea.ac.kr | Jeong, W.K. (BASF Research Center, Kunsan, Republic of Korea)
A promoter-probe shuttle vector pSK1Cat was constructed for the isolation of transcriptional signal sequences from Corynebacterium glutamicum. Besides conferring resistance to kanamycin in Escherichia coli and C. glutamicum, the vector carried a promoterless cat gene to confer resistance to chloramphenicol upon insertion of the appropriate transcriptional signals in the multiple cloning site. By utilizing the vector, a series of transcriptionally active fragments were isolated from the genome of C. glutamicum.
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