Establishment of Mouse Whole Embryo Culture Technique for Detecting Developmental Toxicity in Mice
2004
Kim, J.C. (Chonnam National University, Gwangju, Republic of Korea), E-mail: [email protected] | Son, W.C. (Huntingdon Life Sciences, Alconbury, Huntingdon, UK) | Jiang, Cheng Zhe (Korea Institute of Toxicology, KRICT, Daejeon, Republic of Korea) | Shin, J.Y. (Chonnam National University, Gwangju, Republic of Korea) | Shin, D.H. (Chonnam National University, Gwangju, Republic of Korea) | Jung, N.Y. (Korea Institute of Toxicology, KRICT, Daejeon, Republic of Korea) | Baek, S.S. (Korea Institute of Toxicology, KRICT, Daejeon, Republic of Korea) | Chung, M.K. (Korea Institute of Toxicology, KRICT, Daejeon, Republic of Korea)
The present study was carried out to establish a mouse whole embryo culture technique for detecting developmental toxicity in ICR mice. ICR mouse embryos were explanted on gestational day (GD) 8.5 and cultured for 48 hrs in the bottles containing 1 ml of culture media consisting of 100% immediately centrifuged and heat-inactivated rat serum. The culture bottles with 15 ml capacity were attached to a rotator drum and rotated at 35 rpm, 37.5℃ with a continuous gas flow of 5% O₂, 5% CO₂, 90% N₂for the first 17 hrs; 20% O₂, 5% CO₂, 75% N₂ for the next 17 hrs; 40% O₂, 5% CO₂, 55% N₂ for the last 24 hrs.
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