Expression and localization of phospho-IkB in the central nervous system during experimental autoimmune encephalomyelitis in Lewis rats
2005
Hwang, I.S. (Cheju National University, Jeju, Republic of Korea) | Jee, Y.H. (Cheju National University, Jeju, Republic of Korea), E-mail: yhjee@cheju.ac.kr
To elucidate the roles of phospho-IkB expression in the development and progression of EAE, we investigated the expression of phospho-IkB in the central nervous system (CNS) of rats during experimental autoimmune encephalomyelitis (EAE) using immunohistochemistry and Western blot analysis. In Western blot analysis, the increased expression of phospho-IkB went parallel to severity of EAE. The expression of phospho-IkB increased significantly at the peak stage of EAE followed by gradual decrease. Immunohistochemical studies showed that the phospho-IkB immunoreactivity was mainly expressed in inflammatory cells (macrophages, T cells) and glial cells (astrocytes, microglial cells) at the peak stage of EAE and disappeared at the recovery stage.
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