Mass expression of Apx I and Apx Ⅱ of Actinobacillus pleuropneumoniae in Escherichia coli
2005
Kim, T.J. (Chonnam National University, Gwangju, Republic of Korea) | Lee, B.J. (Chonnam National University, Gwangju, Republic of Korea) | Lee, J.I. (Chonnam National University, Gwangju, Republic of Korea), E-mail: jaeil@chonnam.ac.kr
Actinobacillus pleuropneumoniae is the causative agent of a porcine contagious pleuropneumonia. Among several virulence factors including exotoxin (Apx toxins), LPS, transferrin-binding proteins, OMPs, and some proteases, Apx toxins have been major targets for the protection study. In this study, cloning and expression of A. pleuropneumoniae Apx I and Apx Ⅱ toxin, which are produced by all highly virulent strains, were performed by Escherichia coli expression system. Genes coding Apx I and Ⅱ toxin were amplified from the A. pleuropneumoniae serotype 5 genomic DNA using polymerase chain reaction and cloned to a prokaryotic expression vector, pRSET.
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