Development of the rapid detection kit for Salmonella spp. using immunochromatographic assay
2005
Jung, B.Y. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea), E-mail: jungby@nvrqs.go.kr | Jung, S.C. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea)
An immunochromatographic (IC) strip for the rapid detection of Salmonella spp. in the enriched sample was developed. Affinity purified Salmonella polyclonal antibody was conjugated with 40 nm colloidal gold particles which were prepared by citrate method in our laboratory. The antigen-antibody-gold complex was captured by Salmonella antibody attached to test line of nitrocellulose membrane during the capillary migration of sample. Specificity of the IC strip was calculated to be 100% (12/12) and sensitivity was 97.6% (41/42) in the test with pure cultured bacteria. Salmonella was artificially inoculated into raw pork macerated with enrichment broth.
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